Fermentation conditions
Batch Number GG200120-1  Date 20/01/2020 Operator GG   Settings
 
Strain name Candida boidinii  Medium Xilóz modell oldat  
 
 
 
 
Growth T 30 °C
Carbon source D-Xylose Acceptor D-Xylose Production T  30 °C 
Chromosome  doi: 10.1186/s40793-017-0281-z pH 6 -log[H+]
Plasmids Airflow 100 mL/min
  Stirring 420 rpm
Aim D-Xylitol production
Process G1 Inoc. OD600 9.65 Starting volume [mL] 300 DO   %
Unit jF1 BWM 3.49 End volume [mL] 255 DO2   %
  Flow rate 1  – mL/h
Plate & Inoculums Flow rate 2  – mL/h
  Cell-bank  Streak date Antibiotics Medium  
 
Addition –   
Plate      –    
  Inoculum  Inoc. date Antibiotics Medium Vol  Harvest age (h) Temp (°C) End OD
Inoculum 1    
Inoculum 2 30

 

Growth data & additions
Day hour time (h) Sample OD (600 nm) Dilution factor OD Yxs[g/g] BWM Acid bottle [g] Base added [g] Comment
0 1 0.193 50 9.65 3.48774
15.25 2 0.246 50 12.3 4.61028 0
22.48 3 0.212 50 10.6 3.89016 0.84
40.0 4 0.21 50 10.5 3.8478
46.73 5 0.213 50 10.65 3.91134 2.69
63.55 6 0.219 50 10.95 4.03842 4.21
    70.75 7 0.211 50 10.55 3.86898 4.93
    88.27 8 0.205 50 10.25 3.7419 7.88
    93.77 9 0.285 50 14.25 5.4363

 

Analitical data
    HPLC raw data [g/L]
time (h) Dilution factor Xylose Xylitol AcOH EtOH Ysp[g/g] Xylose Xylitol EtOH AcOH
0 4 7.52583 0.07816 0.07969 0 30.10332 0.31264 0.31876 0
16 4 4.4743 1.7754 0.19369 0.73165 17.8253278276667 7.073081158 0.771648692966667 2.91484726216667
36.6 3.3 3.66887 2.94223 0.30244 1.16362 12.0355885567922 9.65187366122284 0.992142922239333 3.81721117304633
37.05 3.3 0.90298 3.45555 0.47013 2.10918 2.94839410255867 11.28299989047 1.535060045002 6.886856711372
46.05 3.3 0.48325 3.41614 0.55924 2.24153 1.57509960538333 11.1345282274893 1.82278055522933 7.30601762742867
60.5 2 0.2805 5.37998 1.39195 3.054 0.5516117585 10.5798938627267 2.73731189748333 6.005783638
71 2 0.27343 5.0976 1.80963 2.43855 0.536671542483333 10.005254928 3.55183017015 4.78623556275
85.95 2 0.23806 4.66866 2.74634 0.70662 0.465053278328889 9.12028748384 5.36501058727111 1.38039127754667
93.9 2 0.2212 4.55034 2.75702 0.68808 0.431475920026667 8.875959032252 5.37788309688933 1.342178802224

 

Off-line graphs

On-line graphs

 

Observations (fermentation behavior)
General Conclusions
Growth in inoculum



OD and BWM
Lenght of batch phase
Yxs[g/g]
Ysp[g/g]
Aeration/pO2
Quality of broth
Appearance of sample
Other (foaming, pH…)
Technical issue
Human error

Comparison of 

AcOH at 93.9 EtOH at 93.9 Yxs[g/g] growth Yxs[g/g %] production Ysp[g/g %] DO%

 

 

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