| Fermentation conditions | |||||||||
| Batch Number | GG200120-1 | Date | 20/01/2020 | Operator | GG | Settings | |||
| Strain name | Candida boidinii | Medium | Xilóz modell oldat | |
Growth T | 30 | °C | ||
| Carbon source | D-Xylose | Acceptor | D-Xylose | Production T | 30 | °C | |||
| Chromosome | doi: 10.1186/s40793-017-0281-z | pH | 6 | -log[H+] | |||||
| Plasmids | – | Airflow | 100 | mL/min | |||||
| Stirring | 420 | rpm | |||||||
| Aim | D-Xylitol production | ||||||||
| Process | G1 | Inoc. OD600 | 9.65 | Starting volume [mL] | 300 | DO | % | ||
| Unit | jF1 | BWM | 3.49 | End volume [mL] | 255 | DO2 | % | ||
| Flow rate 1 | – | mL/h | |||||||
| Plate & Inoculums | Flow rate 2 | – | mL/h | ||||||
| Cell-bank | Streak date | Antibiotics | Medium | |
Addition | – | |||
| Plate | – | ||||||||
| Inoculum | Inoc. date | Antibiotics | Medium | Vol | Harvest age (h) | Temp (°C) | End OD | ||
| Inoculum 1 | |||||||||
| Inoculum 2 | 30 | ||||||||
| Growth data & additions | |||||||||||
| Day | hour | time (h) | Sample | OD (600 nm) | Dilution factor | OD | Yxs[g/g] | BWM | Acid bottle [g] | Base added [g] | Comment |
| 0 | 1 | 0.193 | 50 | 9.65 | 3.48774 | ||||||
| 15.25 | 2 | 0.246 | 50 | 12.3 | 4.61028 | 0 | |||||
| 22.48 | 3 | 0.212 | 50 | 10.6 | 3.89016 | 0.84 | |||||
| 40.0 | 4 | 0.21 | 50 | 10.5 | 3.8478 | ||||||
| 46.73 | 5 | 0.213 | 50 | 10.65 | 3.91134 | 2.69 | |||||
| 63.55 | 6 | 0.219 | 50 | 10.95 | 4.03842 | 4.21 | |||||
| 70.75 | 7 | 0.211 | 50 | 10.55 | 3.86898 | 4.93 | |||||
| 88.27 | 8 | 0.205 | 50 | 10.25 | 3.7419 | 7.88 | |||||
| 93.77 | 9 | 0.285 | 50 | 14.25 | 5.4363 | ||||||
| Analitical data | ||||||||||
| HPLC raw data | [g/L] | |||||||||
| time (h) | Dilution factor | Xylose | Xylitol | AcOH | EtOH | Ysp[g/g] | Xylose | Xylitol | EtOH | AcOH |
| 0 | 4 | 7.52583 | 0.07816 | 0.07969 | 0 | 30.10332 | 0.31264 | 0.31876 | 0 | |
| 16 | 4 | 4.4743 | 1.7754 | 0.19369 | 0.73165 | 17.8253278276667 | 7.073081158 | 0.771648692966667 | 2.91484726216667 | |
| 36.6 | 3.3 | 3.66887 | 2.94223 | 0.30244 | 1.16362 | 12.0355885567922 | 9.65187366122284 | 0.992142922239333 | 3.81721117304633 | |
| 37.05 | 3.3 | 0.90298 | 3.45555 | 0.47013 | 2.10918 | 2.94839410255867 | 11.28299989047 | 1.535060045002 | 6.886856711372 | |
| 46.05 | 3.3 | 0.48325 | 3.41614 | 0.55924 | 2.24153 | 1.57509960538333 | 11.1345282274893 | 1.82278055522933 | 7.30601762742867 | |
| 60.5 | 2 | 0.2805 | 5.37998 | 1.39195 | 3.054 | 0.5516117585 | 10.5798938627267 | 2.73731189748333 | 6.005783638 | |
| 71 | 2 | 0.27343 | 5.0976 | 1.80963 | 2.43855 | 0.536671542483333 | 10.005254928 | 3.55183017015 | 4.78623556275 | |
| 85.95 | 2 | 0.23806 | 4.66866 | 2.74634 | 0.70662 | 0.465053278328889 | 9.12028748384 | 5.36501058727111 | 1.38039127754667 | |
| 93.9 | 2 | 0.2212 | 4.55034 | 2.75702 | 0.68808 | 0.431475920026667 | 8.875959032252 | 5.37788309688933 | 1.342178802224 | |
Off-line graphs
On-line graphs
| Observations (fermentation behavior) |
General Conclusions | ||||
| Growth in inoculum | |||||
| OD and BWM | |||||
| Lenght of batch phase | |||||
| Yxs[g/g] | |||||
| Ysp[g/g] | |||||
| Aeration/pO2 | |||||
| Quality of broth | |||||
| Appearance of sample | |||||
| Other (foaming, pH…) | |||||
| Technical issue | |||||
| Human error | |||||
Comparison of
| AcOH at 93.9 | EtOH at 93.9 | Yxs[g/g] growth | Yxs[g/g %] production | Ysp[g/g %] | DO% | |