| Fermentation conditions | |||||||||
| Batch Number | GG200120-2 | Date | 20/01/2020 | Operator | GG | Settings | |||
| Strain name | Candida boidinii | Medium | Xilóz modell oldat | |
Growth T | 30 | °C | ||
| Carbon source | D-Xylose | Acceptor | D-Xylose | Production T | 30 | °C | |||
| Chromosome | doi: 10.1186/s40793-017-0281-z | pH | 6 | -log[H+] | |||||
| Plasmids | – | Airflow | 100 | mL/min | |||||
| Stirring | 420 | rpm | |||||||
| Aim | D-Xylitol production | ||||||||
| Process | G1 | Inoc. OD600 | 11.01 | Starting volume [mL] | 300 | DO | % | ||
| Unit | Solida | BWM | 4.30 | End volume [mL] | 255 | DO2 | % | ||
| Flow rate 1 | – | mL/h | |||||||
| Plate & Inoculums | Flow rate 2 | – | mL/h | ||||||
| Cell-bank | Streak date | Antibiotics | Medium | |
Addition | – | |||
| Plate | – | ||||||||
| Inoculum | Inoc. date | Antibiotics | Medium | Vol | Harvest age (h) | Temp (°C) | End OD | ||
| Inoculum 1 | |||||||||
| Inoculum 2 | 30 | ||||||||
| Growth data & additions | |||||||||||
| Day | hour | time (h) | Sample | OD (600 nm) | Dilution factor | OD | Yxs[g/g] | BWM | Acid bottle [g] | Base added [g] | Comment |
| 0 | 1 | 0.367 | 50 | 11.01 | 4.303836 | ||||||
| 15.25 | 2 | 0.212 | 50 | 10.6 | 3.89016 | ||||||
| 22.48 | 3 | 0.199 | 50 | 9.95 | 3.61482 | ||||||
| 40.0 | 4 | 0.215 | 50 | 10.75 | 3.9537 | ||||||
| 46.73 | 5 | 0.176 | 50 | 8.8 | 3.12768 | ||||||
| 63.55 | 6 | 0.216 | 50 | 10.8 | 3.97488 | ||||||
| 70.75 | 7 | 0.198 | 50 | 9.9 | 3.59364 | ||||||
| 88.27 | 8 | 0.213 | 50 | 10.65 | 3.91134 | ||||||
| 93.77 | 9 | 0.249 | 50 | 12.45 | 4.67382 | ||||||
| Analitical data | ||||||||||
| HPLC raw data | [g/L] | |||||||||
| time (h) | Dilution factor | Xylose | Xylitol | AcOH | EtOH | Ysp[g/g] | Xylose | Xylitol | EtOH | AcOH |
| 0 | 4 | 8.3443 | 0.02891 | 0.07807 | 0 | 33.377 | 0.116 | 0.312 | 0.000 | |
| 16 | 4 | 4.56276 | 1.71843 | 0.17072 | 0.73456 | 18.178 | 6.846 | 0.680 | 2.926 | |
| 36.6 | 3.3 | 3.64584 | 3.02444 | 0.28427 | 1.26429 | 11.960 | 9.922 | 0.933 | 4.147 | |
| 37.05 | 3.3 | 1.06326 | 3.57987 | 0.40565 | 1.99975 | 3.472 | 11.689 | 1.325 | 6.530 | |
| 46.05 | 3.3 | 0.61425 | 3.52929 | 0.45576 | 2.01053 | 2.002 | 11.503 | 1.485 | 6.553 | |
| 60.5 | 2 | 0.40648 | 5.54119 | 1.03521 | 2.47949 | 0.799 | 10.897 | 2.036 | 4.876 | |
| 71 | 2 | 0.30661 | 5.41723 | 1.11228 | 1.95572 | 0.602 | 10.633 | 2.183 | 3.839 | |
| 85.95 | 2 | 0.2089 | 5.05476 | 1.16699 | 0.49901 | 0.408 | 9.875 | 2.280 | 0.975 | |
| 93.9 | 2 | 0.19818 | 4.56365 | 0.45682 | 0 | 0.387 | 8.902 | 0.891 | 0.000 | |
Off-line graphs
On-line graphs
| Observations (fermentation behavior) |
General Conclusions | ||||
| Growth in inoculum | |||||
| OD and BWM | |||||
| Lenght of batch phase | |||||
| Yxs[g/g] | |||||
| Ysp[g/g] | |||||
| Aeration/pO2 | |||||
| Quality of broth | |||||
| Appearance of sample | |||||
| Other (foaming, pH…) | |||||
| Technical issue | No pH regulation | ||||
| Human error | |||||
Comparison of
| AcOH at 93.9 | EtOH at 93.9 | Yxs[g/g] growth | Yxs[g/g %] production | Ysp[g/g %] | DO% | |