Composition of reagent 1: Sodium salicylate 120 g/L Sodium citrate 120 g/L Sodium nitroprusside * 2 H20 0.925 g/L Composition of reagent 2: Sodium hydroxide 32 g/L Dichloroisocyanuric acid Sodium salt * 2 H2O 2 g/L Centrifuge 1 mL of culture in an Eppendorf tube at 14’000 for 12 min.  …