The experimental conditions were the same as GG20240831-1, but the media was not sterilized, as we hoped Saccharomyces cerevisiae would outgrow any contaminants due to the low pH. Unsurprisingly, the culture became contaminated early on, and a type of bacteria overgrew the yeast. We used this media to fine-tune the …
AIM: Demonstrating the Superior OTR Capacity of the jFermi Bioreactor: High-Performance Yeast Cultivation on High Sucrose Substrate. Media Preparation: According to Balázs’s suggestion for validating the OTR capacity of the jFermi bioreactor, a Saccharomyces cerevisiae cultivation was performed in a yeast extract and peptone medium with sucrose. To prepare the …
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