Compound name Formula xH2O M [g/mol] C (g/L) Weight Unit Measured weight Batch Number Add
Ammonium sulfate (NH4)2SO4 0 132.14 2 2.000 g      
Disodium hydrogen phosphate Na2HPO4 12 141.96 3.5 8.830 g      
Potassium dihydrogen phosphate KH2PO4 0 136.086 1.5 1.500 g      
Sodium hydrogen carbonate NaHCO3 0 84.007 0.5 0.500 g      
Make QS at 0.8 L Autoclave (121°C, 20 min)
Magnesium sulfate MgSO4 7 120.366 0.2 0.410 g      
Ammonium ferric citrate C6H8FeNO7 2 261.98 0.005 5.688 mg      
Calcium chloride CaCl2 2 110.98 0.01 13.247 mg      
Nickel(II) sulfate NiSO4 7 154.75 0.01 18.149 mg      
Copper(II) sulfate CuSO4 5 159.609 0.01 15.644 mg      
Zinc(II)sulfate ZnSO4 7 161.47 0.01 17.810 mg      
Sodium molybdate Na2MoO4 2 205.92 0.01 11.750 mg      
Manganese(II)sulfate MnSO4 7 151.001 0.005 9.176 mg      
Boric acid H3BO3 0 61.83 0.005 5.000 mg      
Cobalt(II)chloride CoCl2 2 129.839 0.005 6.388 mg      
  100 ml Autoclave (121°C, 20 min)
Fructose C6H12O6 0 180.16 10 10.000 g  
Make QS at with warm water 100 ml Autoclave (121°C, 20 min)

MCN2 minimal medium

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